Agilent, 884973-901, ZORBAX GF-250, 9.4 x 250 mm, 4 µm

ZORBAX GF-250, 9.4 x 250 mm, 4 µm. These legacy USP L35 zirconium-stabilized silica-based SEC columns have a pore size of 150 Å to separate proteins ranging from 4 to 400 kDa molecular weight.

Specifications:
Brand: ZORBAX
Hardware: SS
Inner Diameter (ID): 9.4 mm
LC Platform: Preparative HPLC
Length: 250 mm
Maximum Temperature: 60 °C
Molecular Weight Lower Limit: 4000 Da
Molecular Weight Upper Limit: 400000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisPeptidesProteins
Particle Size: 4 µm
Particle Type: Fully Porous
Phase: GF-250
Pore Size: 150 Å
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 3-8.5

**********The following product description is generated by AI and is for reference only. If you have any questions, please contact customer service.**********
The ZORBAX GF-250 column offers high-performance gel filtration chromatography designed for precise biomolecular separations. Featuring a 9.4 mm internal diameter and a length of 250 mm, this column utilizes 4 micron porous silica particles to ensure excellent resolution and reproducibility when analyzing proteins, peptides, and other macromolecules. Its optimal particle size allows for efficient separation of molecules in the 4 to 250 kDa range, making it ideal for molecular weight determination, protein purity assessment, and aggregate analysis in research and quality control laboratories.

This column is suitable for use with a variety of liquid chromatography systems, including Agilent 1100, 1200, and 1260 Infinity Series HPLC systems, and is compatible with standard aqueous mobile phases commonly used in size exclusion analyses. Typical applications include characterization of monoclonal antibodies, biosimilar comparability studies, and protein-based therapeutic development. The robust packing and chemical stability support consistent performance across multiple runs.

For best results, this column can be paired with ZORBAX guard columns and used alongside Agilent’s range of protein standards and SEC calibration kits. Its reliability and versatility make it a valuable tool for pharmaceutical, biotechnology, and academic research settings requiring accurate macromolecular profiling.