Agilent, GKK-407, DMB Sialic Acid Labeling Kit

DMB Sialic Acid Labeling Kit. Release and derivatization of sialic acids with 1,2-diamino4,5-methylenedioxybenzene (DMB) for separation by liquid chromatography. Comparison may be made against the included sialic acid reference panel (SARP, p/n GKRP-2503), which includes: Neu5Gc (NGNA); Neu5Ac (NANA); Neu5,7Ac2; Neu5Gc,9Ac; Neu5,9Ac2; Neu5,7(8)9Ac3. Up to 20 samples may be processed (2 batches of 10).

Specifications:
Count: 10
Equipment & Reagents Provided by User: Acetonitrile, HPLC gradeGlykoSep R HPLC columnHPLC system for analysisHeating block or oven (set at 50EC for labeling, 80EC for release reaction)Methanol, HPLC gradeWater, HPLC grade
Kit Configuration: Sialic Acid Labeling Kit
Protein Load Range: 1-10 µg

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The DMB Sialic Acid Labeling Kit provides a highly efficient solution for the sensitive detection and quantification of sialic acids in biological samples. Utilizing DMB (1,2-diamino-4,5-methylenedioxybenzene) labeling chemistry, this kit enables fluorescent derivatization of free or released sialic acids to facilitate subsequent analytical separation and detection. The included reagents ensure rapid and reliable labeling, making it suitable for research investigating glycoprotein profiling, biomarker characterization, or disease-related glycosylation changes. After labeling, samples can be analyzed with high sensitivity using HPLC or UHPLC systems equipped with a fluorescence detector, including Agilent’s 1260 Infinity II and 1290 Infinity systems. This workflow is compatible with a range of sample types, such as serum, plasma, tissue extracts, and purified glycoproteins. The kit is commonly used in biopharmaceutical development, clinical research, and structural glycomics studies. For comprehensive glycan analysis, this product integrates seamlessly with Agilent glycan sample preparation kits and analytical columns, providing a streamlined workflow from sample to results. Researchers benefit from enhanced reproducibility, straightforward protocols, and precise quantification of sialic acids for glycan analysis projects.