Agilent, GX96-IPC, AdvanceBio Gly-X N-glycan prep with InstantPC kit, 96-ct

AdvanceBio Gly-X N-glycan prep with InstantPC kit, 96-ct. Complete kit utilizes a novel in-solution enzymatic protein deglycosylation followed by rapid labeling of released N-glycans with InstantPC dye. After a simple cleanup step, the glycan samples are ready for analysis by UHPLC, LC/MS, MS/MS and other methods. With deglycosylation and labeling carried out in solution, the method is simple, rapid, and suitable for automation. The InstantPC dye delivers unmatched fluorescent brightness and MS signal, that enables a single labeling method to be deployed across different glycan analysis workflows. Sample preparation time: ~1 hour. Includes GX96-100 Gly-X deglycosylation module, GX96-101 Gly-X InstantPC labeling module, GX96-102 Gly-X InstantPC cleanup module.

Specifications:
Application: Flexible, high-throughput format:process 1 to 96 samples
Count: 96
Equipment & Reagents Provided by User: 96-well Thermocycler or two independent heat blocks, set for 90°C and 50°C. (e.g. Corning THERM-1001, 110V. THERM-1000, 230V). Note:Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocksVacuum pump (Millipore WP6211560, 110 V, WP6122050, 220V, Welch WOB-L Pump 2522). Note:If you have a vacuum manifold and pump other than the Millipore model suggested, please contact us for information. Alternative vacuum manifold may be used with a GX200 spacer in place of GX100 (see FAQs)
Kit Configuration: Sample Prep Kit
Label: InstantPC
Precision (well-to-well, day-to-day): <5% CV
Prep time (approx): ~1 hour
Preservation of Sialylated Species: > 95 %
Protein Load Range: 1-40 µg
Recovery: > 95 %
Stability: Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF
Technique: LC & LC/MS

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This kit enables streamlined and efficient preparation of N-glycans with integrated fluorescent labeling for high-sensitivity glycan analysis. Leveraging InstantPC technology, it delivers rapid glycan release and labeling in a single, convenient workflow. The 96-count format supports high-throughput sample processing, making it ideal for biopharmaceutical quality control, glycoprofiling, and detailed characterization of therapeutic monoclonal antibodies and other glycoproteins.

Researchers benefit from enhanced detection sensitivity, providing robust results for downstream applications such as liquid chromatography with fluorescence detection LC-FLD, ultra-high performance liquid chromatography-mass spectrometry UHPLC-MS, and capillary electrophoresis systems. The protocol minimizes hands-on time and sample loss, supporting both discovery and routine lot release testing environments.

This solution is compatible with a range of analytical platforms, including Agilent InfinityLab LC systems, AdvanceBio columns, and BioConfirm software, facilitating seamless integration into existing workflows. By offering consistent and reproducible results, it empowers glycomics studies requiring accurate profiling and quantitation of N-glycan structures. The kit includes reagents and detailed instructions for reliable sample preparation, supporting regulatory compliance needs in pharmaceutical and biotechnological research settings.