Agilent, PL1251-3103, PL-SAX 4000Å, 25 x 150 mm, 10 µm

PL-SAX 4000Å, 25 x 150 mm, 10 µm. A strong anion exchange HPLC prep column with fully porous PS/DVB particles. This column can withstand a wide pH range (1-14) and is stable at elevated temperatures

Specifications:
Brand: PL-SAX
Hardware: SS
Inner Diameter (ID): 25 mm
LC Platform: Preparative HPLC
Length: 150 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: OligosProteins
Particle Size: 10 µm
Particle Type: Fully Porous
Phase: SAX
Pore Size: 4000 Å
Pressure Rating: 207 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: 0.1M Sodium Sulfate
Technique: LC
UNSPSC Code: 41115711
pH Range: 1-14

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The PL-SAX 4000Å, 25 x 150 mm, 10 µm column is engineered for high-resolution strong anion exchange chromatography. With a pore size of 4000 Angstroms and 10 micron particle size, it is ideal for the separation and analysis of large biomolecules, including proteins, peptides, and nucleic acids. The durable polymeric base provides excellent chemical and pH stability, supporting robust analytical and preparative workflows.

This column is particularly suited for biopharmaceutical research, protein purification, and academic studies requiring reproducible separation of anionic species. Its high capacity for anionic analytes makes it valuable in charge variant analysis, quality control, and method development for monoclonal antibodies and plasma-derived therapeutics. The dimensions of 25 x 150 mm allow for high sample throughput, making it compatible with medium- to large-scale purification tasks.

Designed for seamless integration with a wide range of liquid chromatography systems from Agilent and other major vendors, this column enables precise and efficient ion exchange processes. It pairs effectively with Agilent’s SEC and other IEX columns to cover a broad spectrum of biomolecular separations. This makes it a versatile tool for method development, routine analysis, and scale-up in research and industrial laboratories.