Agilent, PL1251-3703, PL-SAX 4000Å, 25 x 150 mm, 30 µm

PL-SAX 4000Å, 25 x 150 mm, 30 µm. A strong anion exchange HPLC prep column with fully porous PS/DVB particles. This column can withstand a wide pH range (1-14) and is stable at elevated temperatures

Specifications:
Brand: PL-SAX
Hardware: SS
Inner Diameter (ID): 25 mm
LC Platform: Preparative HPLC
Length: 150 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: OligosProteins
Particle Size: 30 µm
Particle Type: Fully Porous
Phase: SAX
Pore Size: 4000 Å
Pressure Rating: 103 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: 0.1M Sodium Sulfate
Technique: LC
UNSPSC Code: 41115711
pH Range: 1-14

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Designed for high-resolution anion exchange chromatography, this column features a strong anion exchange (SAX) stationary phase with a 4000 angstrom pore size and 30 micron particle size, optimized for the separation of large biomolecules such as nucleic acids, proteins, and glycoconjugates. With dimensions of 25 by 150 millimeters, it is ideal for preparative and semi-preparative workflows that require efficient purification and fractionation of biological samples. The large pore size supports improved mass transfer and accessibility for high molecular weight analytes, making it suitable for research applications including biomolecule characterization, plasmid DNA purification, and the isolation of oligonucleotides or other polyanionic compounds.

Compatible with most preparative liquid chromatography instruments, including Agilent's preparative HPLC systems, this column integrates seamlessly into existing workflow setups. It can be paired with a range of buffers and eluents tailored for anion exchange methods. The robust packing and chemical stability allow for repeated use under demanding conditions typical of biopharmaceutical, biotechnology, and academic research laboratories. Related products include other stationary phases from Agilent, such as strong cation exchange (SCX) and weak ion exchange columns, which provide complementary separation capabilities for complex biomolecular analysis.