BD, 550291, BD Pharmingen™ Purified Rat Anti-Mouse Ly-6G and Ly-6C

Alternative Name: Ly6c, Lymphocyte antigen 6C2; Lymphocyte antigen 6G, Ly6g, Gr-1
Reactivity: Mouse (QC Testing)
Isotype: Rat IgG2b, κ
Immunogen: Not Reported
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
Concentration: 62.5 µg/ml
RRID: AB_393586
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures For optimal indirect immunohistochemical staining, the RB6-8C5 antibody should be titrated (1:10 to 1:50 diluent) and visualized via a three-step staining procedure in combination with Biotin Anti-Rat IgG2b (Cat. No. 550327) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). Alternatively, Anti-Rat Ig HRP Detection Kit (Cat. No. 551013) containing all secondary reagents, may be used. An appropriate isotype control is Purified Rat IgG2b κ Isotype Control, (Cat. No. 559478).