BD, 550405, BD Pharmingen™ Purified Mouse Anti-Human CD152

Alternative Name: CTLA-4; AILIM; Cytotoxic T-lymphocyte protein 4
Reactivity: Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)
Isotype: Mouse BALB/c IgG2a, κ
Immunogen: Human CTLA4 Recombinant Protein
Application: Flow cytometry (Routinely Tested), Immunofluorescence, Immunohistochemistry-frozen, Intracellular staining (flow cytometry) (Tested During Development), Immunohistochemistry-paraffin (Not Recommended)
Concentration: 250 µg/ml
Workshop Number: IX 34
Entrez Gene ID: 1493
RRID: AB_393666
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: The BNI3.1 clone reactive against human CD152 is tested for immunohistochemical staining of acetone-fixed frozen sections. Tissue tested was human spleen and tonsil. The antibody stains activated T- and B-lymphocytes. The isotype control recommended for use with this antibody is purified mouse IgG2a (Cat. No. 550339). For optimal indirect immunohistochemical staining, the BNI3.1 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with polyclonal, biotin conjugated anti-mouse Igs (multiple adsorbed) (Cat. No. 550337) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880).