BD, 550838, BD Pharmingen™ Purified Mouse Anti-Human MLH-1

Reactivity: Human (QC Testing)
Isotype: Mouse IgG1
Immunogen: Human recombinant MLH
Application: Western blot (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen (Tested During Development)
Concentration: 250 µg/ml
RRID: AB_2297859
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: The MLH-1 antibody is recommended to test for immunohistochemical staining of frozen sections and formalin-fixed paraffin embedded sections. Tissues tested were human tonsil, colon carcinoma and intestine. For paraffin sections microwave oven pretreatment with BD Retrievagen A (pH 6.5) (Cat. No. 550524) is required. The staining seen is usually nuclear although some staining may be observed in the cytoplasm. The isotype control recommended for use with this antibody is purified mouse IgG1 (Cat. No. 550878). For optimal indirect immunohistochemical staining, MLH-1 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with polyclonal, biotin conjugated anti-mouse Igs (multiple adsorbed) (Cat. No. 550337) as the secondary antibody and Streptavidin-HRP (Cat.No. 550946) together with the DAB detection system (Cat. No. 550880). Alternatively, the Anti-mouse Ig HRP Detection Kit (Cat. No. 551011) contains all the detection reagents required. For Western blot application of the G168-15 clone reactive against MLH-1, please refer to Cat. No. 551091.