BD, 551051, BD Pharmingen™ Purified Hamster Anti-Human Bcl-2

Reactivity: Human (QC Testing)
Application: Western blot (Routinely Tested), Flow cytometry, Immunofluorescence, Immunohistochemistry-frozen, Immunoprecipitation (Reported)
Regulatory Status: RUO
Description: Description Bcl-2 is considered to be novel among proto-oncogenes because it blocks apoptosis (programmed cell death) in many cell types. Apoptosis is an active form of cellular suicide that typically requires new RNA and protein synthesis and is associated with distinct morphological changes including cell shrinkage, cytoplasm membrane blebbing, nuclear fragmentation and DNA degradation. The Bcl-2 gene was first found in t(14:18) containing follicular B-cell lymphomas. A high proportion of these lymphomas contains t(14:18) chromosomal translocations involving the human Bcl-2 gene. Translocation of Bcl-2 sequences from chromosome 18 onto the transcriptionally active immunoglobulin locus at chromosome band 14q32 in B-cells deregulates Bcl-2 gene expression, resulting in high levels of Bcl-2 mRNA and protein expression. Because Bcl-2 blocks apoptosis it may contribute to tumorigenesis by prolonged cell survival rather than by accelerating the rate of cell proliferation. The reduced molecular weight of Bcl-2 is 26 kDa. Additional minor bands at 27-31 kDa and 18-21 kDa may also be observed.
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store both the hamster anti-human Bcl-2 antibody (component 51-1513GR) and the Jurkat cell lysate (component 51-16526N) undiluted at -20°C.
Recommended Assay Procedures: Recommended Assay Procedures Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml