BD, 551380, BD Pharmingen™ Biotin Rat Anti-Mouse CD51

Alternative Name: Itgav; Integrin alpha-V; Integrin αV ; VNRA; Vitronectin receptor alpha
Reactivity: Mouse (QC Testing)
Isotype: Rat SD, also known as Sprague-Dawley (outbred) IgG1, κ
Immunogen: Mouse (BALB/c) IL-2-activated killer (LAK) Cells
Application: Flow cytometry (Routinely Tested)
Concentration: 0.5 mg/ml
RRID: AB_394174
Storage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Recommended Assay Procedures: Recommended Assay Procedures Flow cytometry: Since this antigen is expressed at low density on the cell surface, it may be desirable to use a second-step reagent conjugated to a "bright" fluorochrome, such as PE Streptavidin (Cat. No. 554061).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.