BD, 553082, BD Pharmingen™ PE-Cy™5 Rat Anti-Mouse CD45

Alternative Name: Ptprc; LCA; Leukocyte common antigen; T200; Ly-5; Lyt-4
Reactivity: Mouse (QC Testing)
Isotype: Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2b, κ
Immunogen: Mouse Thymus / Spleen
Application: Flow cytometry (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_394612
Storage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PE-Cy5 (formerly known as BD Cy-Chrome™) under optimum conditions, and unconjugated antibody and free PE-Cy5 were removed.
Recommended Assay Procedures: Recommended Assay Procedures Flow Cytometry: PE-Cy5 tandem fluorochromes have been reported to bind some classes of human macrophages and granulocytes via Fc receptors, and PE has been reported to bind to mouse B lymphocytes via Fc receptors.  Refer to our support protocols under "Multicolor Flow" at our website http://www.bdbiosciences.com/us/s/resources for more information on mouse and rat flow sample preparation and the uses of Mouse BD Fc Block™.