BD, 553238, BD Pharmingen™ Purified Hamster Anti-Mouse CD3e

Alternative Name: CD3; CD3 epsilon; Cd3e; CD3ε; T3e
Reactivity: Mouse (QC Testing)
Isotype: Syrian Hamster IgG2, κ
Immunogen: Mouse T-cell receptor
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), (Co)-stimulation, Immunoprecipitation (Reported)
Concentration: 0.5 mg/ml
RRID: AB_394727
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures It has been observed that pre-incubation of thymus cell suspensions at 37°C for 2 to 4 hours prior to staining enhances the ability of anti-CD3e and anti-αβ TCR mAbs to detect the T-cell receptor on immature thymocytes. For IHC, we recommend the use of purified 500A2 mAb in our special formulation for immunohistochemistry, Cat. No. 550277. For activation of mouse T lymphocytes by plate-bound anti-CD3e mAb, we recommend BD Biosciences Biocoat™ 96-Well Clear Anti-Mouse CD3 T-Cell Activation Plates, Cat. No. 354720.