BD, 554166, BD Pharmingen™ Purified Mouse Anti-p53

Reactivity: Human, Mouse, Rat, Monkey, Hamster, Chicken, Cow (QC Testing)
Isotype: Mouse IgG1
Immunogen: Human p53 aa. 14-289
Application: Western blot (Routinely Tested), Immunohistochemistry-frozen, Immunoprecipitation (Tested During Development), Immunohistochemistry-paraffin (Not Recommended)
Target Molecular Weight: 53 kDa
Concentration: 0.5 mg/ml
RRID: AB_395280
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures SV40-transformed COS-7 monkey kidney cells (ATCC CRL-1651) or other SV40-transformed cell lines are suggested as positive controls. Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml, for Western blot protocols.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.