BD, 554223, BD Pharmingen™ Purified Mouse Anti-Human DCC

Reactivity: Human (QC Testing)
Isotype: Mouse IgG1
Immunogen: Recombinant Human DCC
Application: Western blot (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required) (Reported)
Target Molecular Weight: 175-190 kDa
Concentration: 0.5 mg/ml
RRID: AB_395314
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Applications include western blot analysis (0.5 - 2.0 µg/ml). IMR-32 (ATCC CCL-127) cells are suggested as a positive control. The antibody has also been published for immunohistochemistry of formalin-fixed, paraffin-embedded tissue sections.  By western blot, DCC-specific antibodies typically identify protein species with molecular weights of approximately 175-190 kDa. Doublets in this range have been reported in brain.  Several smaller immunoreactive species, representing degradation products, cross-reactive species, or DCC forms arising from alternative splicing of DCC mRNA or in vivo processing of the DCC protein may also be identified.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.