BD, 554224, BD Pharmingen™ Purified Mouse Anti-Human Androgen Receptor

Reactivity: Human (QC Testing), Rat (Tested in Development)
Isotype: Mouse IgG1
Immunogen: Purified human androgen receptor recombinant protein
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen, Immunoprecipitation (Tested During Development)
Concentration: 0.5 mg/ml
RRID: AB_395315
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Applications include flow cytometry (titrate between 0.06 and 1 µg/one million cells), immunohistochemical staining of acetone-fixed, frozen sections, and of formalin-fixed, paraffin-embedded tissue sections (5-10 µg/ml) and immunoprecipitation (1-2 µg/one million cells). Clone G122-434 (Cat. No. 554225) is suggested for western blot analysis. LNCaP prostate carcinoma cells (ATCC CRL 1740) are suggested as positive controls.