BD, 554435, BD Pharmingen™ PE Rat Anti-Mouse IL-4

Reactivity: Mouse (QC Testing)
Isotype: Rat IgG1, κ
Immunogen: Partially Purified Mouse IL-4
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_395391
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
Recommended Assay Procedures: Recommended Assay Procedures Flow Cytometry: The 11B11 antibody has been reported to be useful for the immunofluorescent staining and flow cytometric analysis of IL-4-producing cells. The PE-conjugated 11B11 antibody can be used for multicolor flow cytometric analyses to identify and enumerate IL-4 producing cells within mixed cell populations (see right image). For optimal immunofluorescent staining with flow cytometric analysis, this anti-cytokine antibody should be pretitrated (≤ 0.5 µg mAb/million cells).   An approprate isotype control is PE-conjugated R3-34, (Cat. No. 554685). For specific methodology, please visit our web site, www.bdbiosciences.com, and go to the protocols section or the chapter on intracellular staining. BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.