BD, 554556, BD Pharmingen™ PE Mouse Anti-Human LT-α

Alternative Name: TNF-β
Reactivity: Human (QC Testing)
Isotype: Mouse IgG1
Immunogen: Recombinant Human LT-α (TNF-β)
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_395478
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures Immunofluorescent Staining and Flow Cytometric Analysis: The 359-81-11 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify and enumerate LT-α producing cells within mixed cell populations. PE-conjugated 359-81-11 antibody is especially suitable for these studies. The use of a specificity control,  such as one of the following, is suggested: 1) recombinant human TNF-β (Cat No. 554619), 2) unlabeled 359-81-11 antibody (Cat. No. 554554), or 3) mouse IgG1 isotype control, PE-MOPC-21 (Cat. No. 554680). ELISA Detection: The biotinylated 359-81-11 antibody is useful as a detection antibody for a sandwich ELISA for measuring human LT-α  protein levels in cell culture supernatants.  Biotinylated 359-81-11 antibody can be paired with the purified 359-238-8 antibody (Cat. No. 554557) as the capture antibody, with recombinant LT-α (Cat. No. 554619) as the standard. For specific methodology, please visit the protocols section or chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com. Neutralization: The NA/LE™ 359-81-11 antibody has been reported to be useful for neutralization of human LT-α bioactivity.   A suitable NA/LE mouse IgG1 isotype control to match the 359-81-11 antibody is the 107.3 antibody, (Cat. No. 554721).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.