BD, 554571, BD Pharmingen™ PE Rat Anti-Human IL-13

Alternative Name: Interleukin-13; NC30; ALRH; BHR1; P600
Reactivity: Human (QC Testing)
Isotype: Rat IgG1
Immunogen: Human Recombinant IL-13
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_395485
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
Recommended Assay Procedures: Recommended Assay Procedures Immunofluorescent Staining and Flow Cytometric Analysis: The PE Rat Anti-Human IL-13 (Cat. No. 554571) can be used for multicolor immunofluorescent staining and flow cytometric analysis to identify and enumerate IL-13 producing cells within mixed cell populations (see Figure). For optimal immunofluorescent staining and flow cytometric analysis, this anti-cytokine antibody should be titrated (≤ 0.5 µg mAb/million cells). For specific methodology, please visit our website, https://www.bdbiosciences.com/en-us/resources/protocols. A useful control for demonstrating specificity of staining is either of the following: 1) pre-block the PE Rat Anti-Human IL-13 antibody with ligand (e.g., human IL-13) prior to staining, or 2) pre-block paraformaldehyde-fixed/saponin-permeabilized cells with unlabeled Rat Anti-Human IL-13 antibody (e.g., Cat. No. 554570) prior to staining. The intracellular staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe.  A suitable rat IgG1 isotype control immunoglobulin for assessing the level of background staining on paraformaldehyde-fixed/saponin-permeabilized mouse or human cells is the PE Rat IgG1, κ Isotype Control (Cat. No. 554685); use at comparable concentrations to antibody of interest (e.g., ≤ 0.5 µg mAb/1 million cells). BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.