BD, 554730, BD Pharmingen™ PE Mouse Anti-Human MIP-1α (CCL3)

Alternative Name: CCL3
Reactivity: Human (QC Testing), Rhesus, Cynomolgus (Tested in Development)
Isotype: Mouse IgG2a, κ
Application: Intracellular staining (flow cytometry) (Routinely Tested), Immunofluorescence (Tested During Development)
Concentration: 0.2 mg/ml
RRID: AB_395532
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Recommended Assay Procedures: Recommended Assay Procedures The PE Mouse Anti-Human MIP-1α (CCL3) can be used for multicolor immunofluorescent staining and flow cytometric analyses to identify and enumerate MIP-1α (CCL3) producing cells with mixed cell populations. For optimal immunofluorescent staining with flow cytometric analysis, this anti-cytokine antibody should be titrated (≤ 0.5 µg mAb/million cells). A useful control for demonstrating specificity of staining is either of the following: 1) pre-block the conjugated 11A3 antibody with ligand (e.g., recombinant human MIP-1α (CCL3)) prior to staining, or 2) pre-block the fixed/permeabilized cells with unlabeled 11A3 antibody prior to staining. The intracellular staining technique and use of blocknig controls are described in detail by C. Prussin and D. Metcalfe. A suitable mouse IgG2a, κ isotype control immunoglobulin for assessing the level of background staining on paraformaldehyde-fixed/saponin-permabilized human cells is PE Mouse IgG2a, κ Isotype Control (Cat. No. 554648); use at comparable concentrations to antibody of interest (e.g., ≤0.5 µg mAb/1 million cells).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application. Please refer to http://regdocs.bd.com to access safety data sheets (SDS). Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.