BD, 556329, BD Pharmingen™ Purified Mouse Anti-GFAP

Reactivity: Human (QC Testing), Mouse, Rat, Pig, Dog, Chicken, Rabbit, Cow, Guinea Pig, Sheep (Tested in Development)
Isotype: Mouse IgG2b
Immunogen: Bovine spinal cord homogenate
Application: Flow cytometry (Routinely Tested), Immunofluorescence, Immunohistochemistry-formalin (antigen retrieval required) (Tested During Development), Immunohistochemistry-frozen, Western blot (Reported)
Target Molecular Weight: 50 kDa
Concentration: 0.5 mg/ml
RRID: AB_396367
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Pharmingen offers additional GFAP specific antibodies: clone 1B4 (Cat. No. 556328); 4A11 (Cat. No. 556327); clones 1B4, 4A11, 2E1 combined and available as a "cocktail" (Cat. No. 556330). Applications include immunohistochemical staining of formalin-fixed paraffin-embedded brain tissue sections (5-25 µg/ml); and western blot analysis (1-2 µg/ml). Rat brain is suggested as a positive control.