BD, 556371, BD Pharmingen™ Purified NA/LE Mouse Anti-Human CD178

Alternative Name: Fas ligand; FASL; FASLG; CD95 ligand; CD95L; CD95-L; TNFSF6; APTL; APT1LG1
Reactivity: Human (QC Testing)
Isotype: Mouse IgG1
Immunogen: Mouse T lymphoma cells (L5178Y) expressing human FasL
Application: Flow cytometry (Routinely Tested), Immunoprecipitation (Tested During Development), Neutralization (Reported), Western blot (Not Recommended)
Concentration: 1.0 mg/ml
RRID: AB_396392
Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Recommended Assay Procedures: Recommended Assay Procedures NOK-1 has also been shown to neutralize the cytotoxic activity of FasL. Neutralization of FasL activity inhibits Fas-mediated killing. Purified NA/LE Mouse Anti-Human CD178 (Cat. No. 556371) should be used for all functional assays. NOK-1 and a related human FasL clone, NOK-2 [Cat. No. 556376 (purified) and No. 556375 (NA/LE)] may give different profiles in neutralization assays. It is thought that NOK-1 and NOK-2 likely recognize different FasL epitopes. Neither NOK-1 nor NOK-2 are suggested for western blot analysis.