BD, 558508, BD Pharmingen™ Ig Isotype Control Cocktail - B

Application: Flow cytometry (Tested During Development)
Regulatory Status: RUO
RRID: AB_1645631
Description: Description The Isotype Control Cocktail B is a mixture of Mouse IgG1, Mouse IgG2a, and Mouse IgM designed and optimized to work in conjunction with the Rat T Lymphocyte Cocktail (Cat. No. 558493).  The Mouse IgM G155-228 is a monoclonal antibody specific for trinitrophenol (TNP), a hapten not expressed on mammalian cells.  The Mouse IgG2a G155-178 antibody was developed against TNP as well, but has unknown specificity.  The Mouse IgG1 MOPC-21 immunoglobulin is a mouse myeloma protein. All immunoglobulins were selected as isotype controls following screening for low background on a variety of mouse and human tissues.
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
Recommended Assay Procedures: Recommended Assay Procedures BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.