BD Pharmingen™ Alexa Fluor® 488 Rat Anti-Mouse CD125 558533

Alternative Name: Il5r; Il5ra; IL-5RA; IL-5Rα; IL-5R-alpha; IL-5R subunit alpha
Reactivity: Mouse (QC Testing)
Isotype: Rat WI, also known as Wistar (outbred) IgG1, λ
Immunogen: Mouse T88-M Cell Line
Application: Flow cytometry (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_647076
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.
Recommended Assay Procedures: Recommended Assay Procedures Flow cytometry: For staining of cell suspensions from peripheral lymphoid tissues, it is recommended that the cells be pre-incubated with Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) mAb, Clone 2.4G2 (MN 553141).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC). The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR. An isotype control should be used at the same concentration as the antibody of interest. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.