BD, 558774, BD Pharmingen™ Purified Rat Anti-Mouse CD140a

Alternative Name: Pdgfra; Pdgfr2; PDGF-R alpha; Platelet derived growth factor receptor alpha
Reactivity: Mouse (QC Testing)
Isotype: Rat WF, also known as Wistar Furth IgG2a, κ
Immunogen: Mouse PDGF Receptor α chain
Application: Flow cytometry (Routinely Tested), ELISA, Functional assay, Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen, Western blot (Reported)
Concentration: 0.5 mg/ml
RRID: AB_397117
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures This antibody has been tested by immunofluorescent staining (≤ 1 µg/million cells) with flow cytometric analysis to assure specificity and reactivity. Other reported applications include western blot analysis, ELISA, immunohistochemical staining of paraffin-embedded sections, inhibition of ligand binding, and in vivo blocking of PDGF action, but these were not tested at BD.
Product Notices: Product Notices Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Since applications vary, each investigator should titrate the reagent to obtain optimal results. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).