BD, 558937, BD Pharmingen™ PE Mouse Anti-Human CD119

Alternative Name: IFN-γ Receptor α; IFN-γRα; IFNGR1; IFNGR; IFN-gamma receptor 1/IFN-gamma-R1
Reactivity: Human (QC Testing)
Isotype: Mouse IgG2b, κ
Immunogen: Human IFN-γRα
Application: Flow cytometry (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_397165
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Recommended Assay Procedures: Recommended Assay Procedures Immunofluorescent staining and flow cytometric analysis: PE Mouse Anti-Human CD119 (Cat. No. 558937) can be used for the immunofluorescent staining (≤ 1 µg antibody/10[6] cells) and flow cytometric analysis of the levels of membrane IFN-γRα expressed by human cell lines or human lymphoid cells. An appropriate immunoglobulin isotype control is PE Mouse IgG2b, κ Isotype Control (Cat. No. 555058). Since GIR-94 is a non-neutralizing antibody, it can be used for the unobstructed immunofluorescent staining and flow cytometric analysis of cells in systems where the IFN-γ ligand is present. Based on our testing results,(data not shown) the presence of exogenous recombinant human IFN-γ at levels ≤ 50 ng/10[6] cells was insufficient to inhibit the binding of GIR-94 (at 0.06 µg mAb/1 million cells).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.