BD, 559749, BD Pharmingen™ Purified Rat Anti-Mouse Ig, κ Light Chain

Reactivity: Mouse (QC Testing)
Isotype: Rat SD, also known as Sprague-Dawley (outbred) IgG1, κ
Immunogen: Mouse IgG2b κ secreted by MPC-11 plasmacytoma
Application: ELISA, Flow cytometry (Routinely Tested), Intracellular staining (flow cytometry) (Tested During Development)
Concentration: 0.5 mg/ml
RRID: AB_397313
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures For the sandwich mouse IgG1, G2a, G2b, G3, IgM, IgA, and IgE ELISA, biotin-conjugated mAb 187.1 (Cat. No. 559750, cocktailed with biotin-conjugated anti-mouse Ig λ1, λ2, and λ3 light chain, R26-46 mAb, Cat. No. 553433) is optimal for detection with anti-mouse Ig isotype specific mAbs (A85-3, Cat. No. 553445; R19-15, Cat. No. 553387; R12-3, Cat. No. 553392; R2-38, Cat. No. 553404; II/41, Cat. No. 553435; C10-1, Cat. No. 556960; C10-3, Cat. No. 556969; R35-72, Cat. No. 553413) for capture, respectively. For flow cytometric detection of intracytoplasmic Ig, κ light chain, we recommend FITC-conjugated mAb 187.1 (Cat. No. 550003). Since applications vary, each investigator must determine dilutions appropriate for individual use.