BD, 560788, BD Phosflow™ PE Mouse anti-CrkL (pY207)

Reactivity: Human (QC Testing), Mouse, Rat (Predicted)
Isotype: Mouse BALB/c IgG2a, κ
Immunogen: Phosphorylated Human, Mouse, and Rat CrkL Peptide
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Vol. Per Test: 20 µl
RRID: AB_1937309
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Recommended Assay Procedures: Recommended Assay Procedures This PE-conjugated antibody is suitable for intracellular staining of cell lines using BD Cytofix™ Fixation Buffer and BD Phosflow™ Perm Buffer III or IV. We have confirmed that the Alexa Fluor® 647 conjugate (Cat. No. 560790) is suitable for staining leukocytes from human peripheral blood and bone marrow.