BD, 562077, BD Phosflow™ PE Mouse Anti-Stat5 (pY694)

Alternative Name: Signal transducer and activator of transcription 5; MGF; MPF
Reactivity: Human (QC Testing), Mouse, Rat, Sheep (Predicted)
Isotype: Mouse IgG1, κ
Immunogen: Phosphorylated Human Phosphorylated Stat5 Peptide
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Vol. Per Test: 5 µl
RRID: AB_10894188
Storage Buffer: Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Recommended Assay Procedures: Recommended Assay Procedures This antibody conjugate is suitable for intracellular staining of human whole blood (using BD Phosflow™ Lyse/Fix Buffer) and peripheral blood mononuclear cells (using BD Cytofix™ Fixation Buffer or BD Phosflow™ Fix Buffer I). This mAb was characterized by flow cytometry (Flow) and western blot analysis (WB) using these model systems: Method Species Cells Treatment Fixation Perm buffer Result Flow Human PBMC IL-2 Fixation Buffer III Positive Staining Flow Human PBMC IL-2 Fixation Buffer I or II Unsatisfactory Flow Human Whole Blood IL-2 Lyse/Fix III Positive Staining Flow Human Whole Blood IL-2 Lyse/Fix I or II Unsatisfactory Flow Human TF-1 cells GM-CSF Fixation Buffer III Positive Staining Flow Human TF-1 cells GM-CSF Fixation Buffer I or II Unsatisfactory WB Human A431 Cell Lysate EGF Not Applicable Not Applicable 92 kDa