BD, 562479, BD Pharmingen™ Alexa Fluor® 488 Mouse anti-EZH2

Alternative Name: Enhancer of zeste 2; ENX-1; Histone-lysine N-methyltransferase EZH2; KMT6
Reactivity: Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)
Isotype: Mouse IgG1
Immunogen: Human EZH2 recombinant protein aa. 156-256
Application: Intracellular staining (flow cytometry) (Routinely Tested), Bioimaging, Immunofluorescence (Tested During Development)
Vol. Per Test: 5 µl
Entrez Gene ID: 2146, 14056, 312299
RRID: AB_11151903
Storage Buffer: Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.
Recommended Assay Procedures: Recommended Assay Procedures • This antibody conjugate is suitable for intracellular staining of human cell lines using BD Cytofix™ Fixation Buffer. BD Phosflow™ Perm/Wash Buffer I (Cat. No. 557885) and BD Phosflow™ Perm Buffer III (Cat. No.558050) can  be used with this antibody conjugate. • The Bioimaging protocol can be found at http://www.bdbiosciences.com/support/resources/protocols/ceritifed_reagents.jsp.