BD, 565806, BD Horizon™ BV421 Mouse Anti-Human CD11c

Alternative Name: ITGAX; AlphaX Integrin; Axb2; Integrin alpha-X; CR4; SLEB6; p150,95 alpha
Reactivity: Human (QC Testing), Rhesus (Tested in Development)
Isotype: Mouse IgG1, κ
Immunogen: Human monocytes and synovial cells
Application: Flow cytometry (Routinely Tested), Immunofluorescence (Tested During Development)
Concentration: 0.2 mg/ml
Workshop Number: III 278; IV M66
RRID: AB_2869718
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
Recommended Assay Procedures: Recommended Assay Procedures Note: The binding of the 3.9 antibody to CD11c is divalent cation dependent. Therefore, heparin is recommended for use as the blood anticoagulant rather than the EDTA chelating agent that might adversely affect 3.9 antibody binding and cellular staining. For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).