BD, 566352, BD Horizon™ Human CD4 Fluorochrome Evaluation Kit

Concentration: 50 µg/ml
Application: Flow cytometry (Routinely Tested)
Regulatory Status: RUO
RRID: AB_2869752
Description: Description The Human CD4 Fluorochrome Evaluation Kit includes 10 µg of the following components: • Alexa Fluor® 700 Mouse Anti-Human CD4 • BV480 Mouse Anti-Human CD4 • APC Mouse Anti-Human CD4 • BV510 Mouse Anti-Human CD4 • APC-Cy™7 Mouse Anti-Human CD4 • BV605 Mouse Anti-Human CD4 • APC-R700 Mouse Anti-Human CD4 • BV650 Mouse Anti-Human CD4 • BB515 Mouse Anti-Human CD4 • BV711 Mouse Anti-Human CD4 • BB700 Mouse Anti-Human CD4 • BV750 Mouse Anti-Human CD4 • BUV395 Mouse Anti-Human CD4 • BV786 Mouse Anti-Human CD4 • BUV496 Mouse Anti-Human CD4 • PE Mouse Anti-Human CD4 • BUV563 Mouse Anti-Human CD4 • PE-Cy™5 Mouse Anti-Human CD4 • BUV661 Mouse Anti-Human CD4 • PE-Cy™7 Mouse Anti-Human CD4 • BUV737 Mouse Anti-Human CD4 • PE-CF594 Mouse Anti-Human CD4 • BUV805 Mouse Anti-Human CD4 • PerCP-Cy™5.5 Mouse Anti-Human CD4 • BV421 Mouse Anti-Human CD4 • FITC Mouse Anti-Human CD4 • BUV615 Mouse Anti-Human CD4 • R718 Mouse Anti-Human CD4 • RY586 Mouse Anti-Human CD4 • RB780 Mouse Anti-Human CD4 • RB705 Mouse Anti-Human CD4 • RB545 Mouse Anti-Human CD4 • RB744 Mouse Anti-Human CD4 • RB613 Mouse Anti-Human CD4 • RY610 Mouse Anti-Human CD4 • RY703 Mouse Anti-Human CD4 • RY775 Mouse Anti-Human CD4 To access the Technical Data Sheet (TDS) for individual conjugates, search the name of the clone and/or the specificity on the website: http://www.bdbiosciences.com Fluorochrome brightness is representative of the specific Anti-Human CD4 clone, other clones may differ. As for all tandem reagents, spillover values should not be assumed to be identical for other clones. Please note that the following dyes are detected in the same channel.  To access the Absorption and Emission Spectra for each individual fluorochrome, go to: https://www.bdbiosciences.com/en-us/resources/bd-spectrum-viewer • FITC, BB515, RB545 • BV510, BV480 • BB700, PerCP-Cy5.5 • Alexa Fluor® 700, APC-R700, R718 • PE, RY586 • PE-Cy7, RB780 (when using blue laser)
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated under optimal conditions that minimize unconjugated dye and antibody. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application. For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385). Note:  When using high concentrations of antibody, background binding of this dye to erythroid cell subsets (mature erythrocytes and precursors) has been observed.  For researchers studying these cell populations, or in cases where light scatter gating does not adequately exclude these cells from the analysis, this background may be an important factor to consider when selecting reagents for panel(s).