BD, 567021, BD Pharmingen™ Purified Rat Anti-Mouse CD16/CD32

Alternative Name: Fcgr3/Fcgr2b; Fc gamma RIII/Fc gamma RIIB; FcγRIII/FcγRIIB
Reactivity: Mouse (QC Testing)
Isotype: Rat WI, also known as Wistar (outbred) IgG2a, λ
Immunogen: Mouse Pre-B cells
Application: Flow cytometry (Routinely Tested)
Concentration: 0.5 mg/ml
Entrez Gene ID: 14130, 14131
RRID: AB_2870011
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Fc Receptor Blocking for Flow Cytometric Analysis To reduce Fc receptor-mediated binding by antibodies of interest to cells that express CD16 and/or CD32 for flow cytometric analysis: 1. Preincubate cell suspension with Purified Ab93 antibody (Cat. No. 567021) at ≤ 1 μg of Ab93/million cells in 100 μl) at 4˚C for 5 minutes. 2. Add antibody of interest directly to cells preincubated with Purified Ab93 antibody (ie, the Purified Ab93 antibody is not washed away before staining cells). 3. If an Anti-Ig second-step antibody is being used, the secondary reagent must be chosen so that it does not bind to (crossreact with) Purified Ab93 antibody which has a Rat IgG2a, λ isotype. BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBead to ensure that BD CompBeads are appropriate for your specific cellular application.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. Please refer to http://regdocs.bd.com to access safety data sheets (SDS). Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.