Bio-Rad, 1633011, ProteoMiner™ Sequential Elution Large-Capacity Kit

10 preps, protein preparation kit for sequential elution into 4 fractions, for 50 mg of total protein, includes 10 spin columns, buffers, collection tubes
Overview
Choose the ProteoMiner Sequential Elution Large-Capacity Kit to simultaneously concentrate low-abundance proteins and lower the concentration of high-abundance proteins, and fractionate proteins within the enriched population by sequential elution. This kit is designed for 50 mg samples. Protein complexity of the sample is maintained.
The enrichment protocol uses a large, highly diverse library of combinatorial peptide ligands bound to beads. After high-abundance proteins have saturated all their high-affinity ligands, the unbound excess protein is washed away. Most medium- and low-abundance proteins will not saturate their ligands, resulting in no excess to be washed away. After washing the ProteoMiner Sequential Elution Reagents can then be used to fractionate the retained proteins. The elution kit contains four different elution buffers, providing multiple options for successive elution of populations of proteins with different characteristics.
• Compatible with a wide range of sample types
• Four different elution buffers, providing multiple options for successive elution of populations of proteins with different characteristics
• Can be used for differential expression analysis
• Sequential elution has been optimized for samples for SELDI; eluted fractions are not compatible with direct use of fractions in 2-D gel electrophoresis
Kit Contents
• Spin columns, 10; 500 µl bead slurry (20% beads, 20% v/v EtOH), 100 µl settled bead volume
• Wash Buffer, 150 mM NaCl, 10 mM NaH 2 PO 4 , pH 7.4, 50 ml
• Elution Reagent, lyophilized 8 M urea, 2% CHAPS, 5 vials
• Rehydration Reagent, 5% acetic acid, 5 ml
• Elution Reagent 1, 1 M sodium chloride, 20 mM HEPES, pH 7.4, 5 ml
• Elution Reagent 2, 200 mM glycine, pH 2.4, 5 ml
• Elution Reagent 3, 60% ethylene glycol, 5 ml
• Elution Reagent 4, 33.3% 2-propanol, 16.7% acetonitrile, 0.1% trifluoroacetic acid, 5 ml
• Plasma Preparation Buffer, 1 M sodium citrate, 20 mM HEPES, pH 7.4, 1 ml
• Capless 2 ml centrifuge tubes, 20
• Capped 2 ml centrifuge tubes, 40
• Instruction Manual
• Quick Start Guide
Product Options
Related Products
• Mini Bio-Spin ® Chromatography Columns ( 7326207 )
• ReadyPrep™ Proteomics Grade Water ( 1632091 )
• ReadyPrep 2-D Cleanup Kit
Related Categories
• Centrifuges
• Protein Electrophoresis and Blotting
• 2-D Electrophoresis
• Protein Assay Kits and Cuvettes
• Protein Fractionation
• Magnetic Beads and Racks for Immunoprecipitation
Supporting Documentation
• ProteoMiner Protein Enrichment and Sequential Kit Protocols
• ProteoMiner Sequential Elution Kit Protocol
• ProteoMiner Protein Enrichment Kits Instruction Manual
• ProteoMiner Protein Enrichment Technology Brochure
• Enrichment of Medium- and Low-Abundance Proteins in Sample Types Using ProteoMiner Technology Application Note