Cytiva, 17127901, rProtein A Sepharose™ Fast Flow antibody purification resin

rProtein A Sepharose Fast Flow resin, 5 mL rProtein A Sepharose 4 Fast Flow is an affinity resin for antibody purification at laboratory and process scale. • For monoclonal and polyclonal antibody purification at both laboratory and process scale. • Well established among Protein A resins as excellent for monoclonal antibody (mAb) processes. • Free from animal-derived components. • Hydrophilic base matrix ensures low levels of non-specific binding and low levels of host cell-derived impurities in the elution pool. • Fulfills industrial demands for security of supply, robust performance, and regulatory support. Monoclonal and polyclonal antibody purification rProtein A Sepharose 4 Fast Flow is built upon the well-established Sepharose Fast Flow platform and is used in many approved monoclonal antibody processes. It is composed of recombinant protein A coupled to the cross-linked 4% agarose base matrix.The highly crosslinked agarose resin enables high flow rates and high productivity. Protein A resin characteristics The recombinant protein is produced in E. coli and engineered with an oriented coupling to enhance binding capacity. Epoxy-based coupling ensures low ligand leakage. Specificity of the recombinant protein for the Fc region of IgG is similar to the native protein, giving a high degree of selectivity and yield. Streamlined scale up Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, making purification scale-up straightforward. Successful use in published research Cytiva's protein A resins are used in a variety of applications, including: Molecular characterization of an arthritogenic collagen peptide interacting with I-Ar Compatible empty columns – research scale Cytiva's solutions include a variety of empty columns for packing. rProtein A Sepharose resin is compatible for use with HiScale, Tricorn, and XK columns. Compatible empty columns – bioprocess scale For process-scale applications, Cytiva recommends AxiChrom columns. rProtein A Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow. Convenient prepacked columns – research scale rProtein A Sepharose Fast Flow is also available in convenient prepacked formats: rProtein A GraviTrap, for use with gravity flow HiTrap rProtein A FF, for use with a syringe, pump, or chromatography system Convenient prepacked columns – bioprocess scale Prepacked ReadyToProcess columns are available on request, eliminating the need for column packing and cleaning validation.