Cytiva, OAPMPUNV, Minimate™ tangential flow filtration system

Minimate EVO TFF system package Minimate™ tangential flow filtration system, a Pall Life Sciences product, is designed for highly reliable buffer exchange or concentration of samples up to 1 liter. • Concentrate and desalt proteins, peptides, or nucleic acids (DNA, RNA, oligonucleotides) • Recover antibodies or recombinant proteins from clarified cell culture media • Process metal sensitive enzymes and molecules • Separate (fractionate) large from small biomolecules • Recover or remove viruses from solutions • Prepare samples prior to column chromatography • Concentrate samples after gel filtration What comes with the Minimate™ system? The system package Includes peristaltic pump, pump head, 2 pressure gauges, reservoir, stir plate, drip tray, and assorted fittings. Specifications - Materials of Construction Reservoir: Polysulfone
Reservoir Cover: Polypropylene
Reservoir O-Ring: Silicone
Magnetic Stir Bar: PTFE coated
Gauge Wetted Parts: Type 316L stainless steel
SS Fitting O-ring: EPDM rubber
Gauge Mounting Block: Polypropylene
Luer Fittings: Polypropylene and stainless steel
Tubing: PharMed #16
Stopcocks/valves: PVDF
Drip Tray: Acetal with T304 stainless steel brackets Includes Peristaltic pump, pump head pressure gauge, valves, reservoir with stir bar, built-in stir plate on a drip tray Dimensions 32.6cm W × 48.2cm D × 22 cm H  (12.9 in W × 19 in D × 8.7 in H)​ Weight 8 kg (17.7 lbs) Maximum Inlet Pressure 30 psi Operating Temperature Range 5-50 °C (41-122 °F)​ Approximate Recirculation Flow Rate 10 - 240 mL/min Minimum System Working Volume 15 mL Applications Concentrate and desalt proteins, peptides, or nucleic acids (DNA, RNA, oligonucleotides)
Recover antibodies or recombinant proteins from clarified cell culture media
Process metal sensitive enzymes and molecules
Separate (fractionate) large from small biomolecules
Concentrate viruses or gene therapy vectors
Prepare samples prior to column chromatography
Concentrate samples after gel filtration
Depyrogenate water, buffers, and media solutions Performance Designed to work with the Minimate™ TFF Capsule, this easy-to-use system can process sample volumes up to 1 L or more and efficiently concentrate samples to as little as 5 mL. Subsequent desalting or buffer exchange steps can be run on the same system with minimal user intervention. The ready-to-use Minimate™ TFF System includes a Masterflex L/S variable speed peristaltic pump, Masterflex L/S Easy-Load pump head, as well as a 0 - 4 bar (0 - 60 psi) pressure gauge, valves, 500 mL reservoir with magnetic stir bar, and stir plate all assembled on a compact drip tray. The use of a peristaltic pump with disposable tubing and fittings minimizes sample cross contamination while providing gentle processing for biomolecules. The reservoir is designed to hold up to 500 mL of sample and provides efficient mixing of product. The tapered bottom and optimally located feed and return ports reduce the system hold-up volume, which allows high concentration factors to be achieved. The reservoir lid seals tightly allowing additional sample volume or diafiltration solution to be drawn directly into the reservoir without the need of a transfer pump. Vacuum is created as filtrate is generated through the TFF device allowing continuous diafiltration to be performed. When it's time to retrieve your sample, simply open the valve on the reservoir outlet and pump out your sample. Masterflex and any other third-party trademarks are the property of their respective owners.