Merck, 11644807001, Cell Proliferation Reagent WST-1

Cellular proliferation requires the replication of genomic DNA. Thus, monitoring DNA synthesis is an indirect parameter of cell proliferation, as well as being suitable for the study of the regulation of DNA synthesis. [³H]-thymidine has traditionally been used to label the DNA of replicating (cycling) cells. To circumvent the disadvantages associated with the use of the [³H]-thymidine, nonradioactive alternatives based on 5-bromo-2′-deoxyuridine (BrdU) have been developed. The use of chemiluminescence technology provides enhanced sensitivity and a broad measurement range.