Qiagen, 19101, RNase A

2.5 ml (100 mg/ml; 7000 units/ml, solution)

Features

- Selectively cleaves single-stranded RNA
- Active under a wide range of reaction conditions
- DNase-free and quality-controlled
- Does not need to be boiled before use

Product Details

RNase A is an endoribonuclease that breaks down single-stranded RNA molecules by hydrolysis 3' of pyrimidine residues (cytosine and uracil). RNase A is a basic protein of 124 amino acids, with a molecular weight of approximately 13.7 kDa. RNase A catalyzes the transphosphorylation and degradation of RNA by cleaving specifically at several sites on a single stranded RNA polynucleotide chain at the 3′-side of pyrimidine (uracil or cytosine) phosphate bonds. Ribonucleases like RNase A do not hydrolyze DNA because the DNA lacks 2′-OH groups essential for forming cyclic intermediates. RNase A is widely used to remove contaminating RNA during the isolation of plasmid and genomic DNA.

DNase-free Ribonuclease A is quality-controlled and ready to use for all applications where digestion of RNA is required.

Performance

Feature: RNase A
Quantity: 2.5 mL
Concentration: 100 mg/; 7000 units/ml, solution
Unit definition: One Kunitz unit is the amount of enzyme causing the hydrolysis of RNA at a rate such that the velocity constant (k) equals unity at 25°C and pH 5.0

QIAGEN Ribonuclease A is DNase-free and quality-controlled for use in plasmid purification procedures for the digestion of RNA.

RNase A is widely used to remove contaminating RNA during the isolation of plasmid and genomic DNA. RNase A is also used to remove contaminating RNA from DNA samples after extraction or PCR, especially if downstream applications (e.g., cloning or sequencing) require RNA-free DNA.

RNase A is very active under a wide range of reaction conditions and is difficult to inactivate.

QIAGEN RNase A does not need to be boiled before use.

Procedure

Before use in plasmid and DNA purification, centrifuge QIAGEN RNase A briefly, and then add into Buffer P1 to obtain a final concentration of 100 μg/mL. Store Buffer P1 at 2–8°C after addition of RNase A. For detailed instructions on RNase A usage during plasmid and DNA purification, please refer to the corresponding kit handbook.

Applications

- Plasmid and genomic DNA preparation
- Removal of RNA contamination from recombinant protein preparations
- Cleanup of RNA from enzymatic reactions