Qiagen, 32943, cis-Repressed pQE Kan Vector Set

25 ug each: pQE-80-L-Kan, pQE-81L-Kan, pQE-82L-Kan

Features

- Tightly regulated expression leading to enhanced stability of cytotoxic constructs
- No requirement for second plasmid encoding repressor in trans

Product Details

The vectors in the cis-Repressed pQE Vector Set are similar to those in the N-Terminus pQE Vector Set, but additionally express the laclq gene product that represses protein expression prior to IPTG induction. This eliminates the need to include this gene on another plasmid. Use of these vectors is recommended when expressing gene products toxic to E. coli.

Performance

The cis-Repressed pQE Kan Vector Set contains vectors with a kanamycin resistance gene in addition to the ampicillin resistance gene providing selection by either antibiotic.

Principle

pQE vectors provide high-level expression of protein in E. coli and add the small 6xHis affinity tag to protein expression constructs.

Procedure

Once a protein-encoding DNA insert has been incorporated into the vector, it is transformed into E. coli cells and protein expression is induced by the addition of IPTG to the growth medium. After cell lysis, 6xHis-tagged proteins are purified by Ni-NTA affinity chromatography under native or denaturing conditions.

Applications

- Purification of functional proteins under native conditions
- Purification under denaturing conditions for antibody generation
- Efficient detection using Anti·His antibodies and conjugates

The pQE vectors in this product provide high-level expression of protein in E. coli , enabling: