Qiagen, 931636, QIAsymphony RNA Kit

For as many as 192 preps, depending on protocol: includes 2 reagent cartridges and enzyme racks and accessories

Features

- Prefilled reagent cartridges for ease of use
- High reproducibility through standardized processing
- Optimized protocols provide high yields of pure RNA
- Bar codes enable tracking of samples and reagents

Product Details

The QIAsymphony RNA Kit enables automated purification of total RNA, or total RNA including miRNA and other small RNAs, from 1–96 samples on the QIAsymphony SP . Total RNA can be purified from animal cells, tissues, or FFPE tissues, using very small amounts of starting material (≥100 cells) up to 1 x 10 7 cells or 50 mg of tissue. Optimized protocols provide high yields of pure total RNA, or total RNA including miRNA, and flexible elution volumes allow RNA concentration to be easily optimized for each downstream application. Prefilled reagent cartridges reduce setup time and minimize manual handling. Bar code reading enables full tracking of reagents.

Procedure

Protocol: Running with
RNA CT 400: QIAsymphony RNA Kit (2 x 96) ‡
RNA CT 800: QIAsymphony RNA Kit (2 x 48) ‡
RNA FT 400*: QIAsymphony RNA Kit (2 x 96) ‡
miRNA CT 400*: QIAsymphony RNA Kit (2 x 96) ‡
RNA FFPE 130 †: QIAsymphony RNA Kit (2 x 72) ‡

Innovative, ready-to-run reagent cartridges are prefilled with magnetic particles and all reagents required for the purification procedure, including accessory enzymes. The cartridges are automatically opened by the QIAsymphony SP helping to minimize the risk of environmental contamination (see figure " Prefilled, sealed reagent cartridges "). Worktable setup is rapid, which saves your valuable time. Simply place up to 2 reagent cartridges in the consumables drawer. The reagent cartridges can be either from the same kit or from different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent volumes only for the selected number of samples are used, giving you complete cost control. The purification procedure comprises 4 steps: lyse, bind, wash, and elute. A number of protocols is available for the QIAsymphony SP for purifying RNA, depending on the starting material and the required RNA product (see table).

Applications

The QIAsymphony RNA Kit, and fully automated purification of total RNA on the QIAsymphony SP, provides high-quality RNA suitable for direct use in any downstream application.