Qiagen, RP85T, TaqNova Master Mix 2x (100 U)

100 rxns Components: 2 x 1.25 mL of TaqNova Master Mix 2x, 2 x 1.5 mL PCR-grade water. Formulation: 2x concentrated PCR reaction buffer, 4 mM MgCl2; 1.6 mM dNTPs mix (0.4 mM of each dNTP); 0.04 U/μL TaqNova DNA Polymerase, red inner dye and density reagent. Store all components at -20°C. After thawing, the product will remain stable at 4°C for at least two months. Up to 10 freeze cycles will not compromise product performance.
Show more

Features

- Recombinant enzyme of high purity with a half-life of 45 minutes at 95°C
- Amplifies fragments of up to 5 kb
- Leaves ´A´ overhangs
- Facilitates high throughput PCR reaction setup with a reduced risk of contamination

Product Details

2x PCR TaqNova-RED is a 2x concentrated, ready-to-use PCR master mix that facilitates an easy and rapid PCR reaction setup. The 2x PCR TaqNova-RED solution contains a reaction buffer, magnesium chloride, dNTPs and a thermostable Taq DNA polymerase.

It is supplied with 20 mM Tris-HCl (pH 8.0 at 25°C), 200 mM KCl, 0.1 mM EDTA, 2 mM DTT, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20, 50% (v/v) glycerol.

One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTPs to an insoluble DNA fraction in 30 minutes at 75°C in a 50 μL reaction.

Performance

Assay: Specification
Functional activity: Passed
Unspecific nuclease contamination: None detected

Principle

To set up the PCR reaction, all that needs to be added to the master mix is the template, the primer set and water. This convenient 2x PCR TaqNova-RED master mix reduces the time required to set up PCR reactions and decreases the possibility of contamination, particularly when preparing large volumes of reactions. Moreover, the master mix includes an inner dye and a density reagent, which allows the direct loading of PCR products into a gel.

Procedure

Quality Control

The master mix is free of unspecific nucleases contamination and is extensively tested for PCR applications.

Applications

- Amplification of short and medium size DNA sequences
- Routine and diagnostic PCR
- TA cloning
- Multiplex PCR assays

This is used for standard and specialized applications such as: