Thermo Fisher, 53027, Pierce™ FITC Antibody Labeling Kit

Pierce™ FITC Antibody Labeling Kit The Thermo Scientific FITC Antibody Labeling Kit is a multi-component kit containing a high-performance derivative of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.
Features of the kit:
•Easy—convenient kit with FITC to label and purify antibody in about one hour
•Amine-specific labeling—the isothiocyanate variety of fluorescein efficiently labels antibodies and other purified proteins at primary amines (lysine side chains)
•Optimized kit procedure—following the standard protocol results in antibodies with excellent dye:protein ratios for optimum activity and fluorescence
•Single-use fluors—no need to weigh tiny amounts of powder; kits contain single-use vials of reagent
•Efficient purification—kits include purification resin and easy-to-use spin columns, ensuring rapid and efficient removal of non-reacted dye and excellent protein recoveryProperties of FITC:
• Alternative names: 5/6-FITC
• Chemical name: 5(6)-fluorescein isothiocyanate mixed isomer
• Molecular weight: 389.
2
• Excitation source: 488 nm spectral line, argon-ion laser
• Excitation wavelength: 494 nm
• Emission wavelength: 518 nm
• Extinction coefficient: > 70,000 M-1cm-1
• CAS #: 27072-45-3
• Purity: > 95% by HPLC
• Solubility: Soluble in aqueous buffers at pH > 6
• Reactive groups: Isothiocyanate, reacts with primary amines at pH 7.
0 to 9.
0Applications:
• Label antibodies for use as immunofluorescent probes
• Label oligonucleotides for hybridization probes
• Detect proteins in gels and on Western blotsAmine-reactive Fluorescein DyesFITC is the base fluorescein molecule functionalized with anisothiocyanatereactive group (—N=C=S) at one of two hydrogen atoms on the bottom ring of the structure. This derivative is reactive towards primary amine groups on proteins, peptides and other biomolecules. NHS-fluorescein is activated with theN-hydroxy-succinimidyl-ester (NHS ester) functional group. Compared to FITC, the NHS-ester deriviative has greater specificity toward primary amines in the presence of other nucleophiles and results in a more stable linkage following labeling. Pierce Amine-reactive Fluorescein Dyes are mixtures of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra, and for protein applications there is no need to isolate a specific isomer.
Description: Pierce FITC Antibody Labeling Kit
Excitation/Emission: 494/518
Labeling Scale: 0.
5 to 1 mg
Product Line: Pierce™
Product Type: Labeling Kit
Quantity: 3 reactions kit
Sufficient For: Labeling and purifying 3 x 1 mg (2 mg/mL) of IgG or other proteins
Labeling Target: Antibodies, Proteins
Label or Dye: FITC (Fluorescein)
Solubility: Aqueous Buffer
Unit Size: Each