Thermo Fisher, FNN0011, Cell Extraction Buffer

Thermo Fisher, FNN0011, Cell Extraction Buffer Cell Extraction Buffer is a ready-to-use lysis buffer for use in ELISA and western blotting applications for total protein extraction from mammalian cells. The buffer uses detergent-based lysis, eliminating the need for mechanical cell disruption, providing a milder and easier alternative when isolating proteins from cell cultures. The formulation helps retain the protein structure and function needed for enzyme assays or immunoassays.
Buffer formulation:10 mM Tris, pH 7.
4, 100 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1 mM NaF, 20 mM Na4P2O7,2 mM Na3VO4, 1% Triton X-100, 10% glycerol, 0.
1% SDS, 0.
5% deoxycholateThis cell extraction buffer does not contain protease inhibitors and should be supplemented with 1 mM PMSF andprotease inhibitor cocktailjust prior to use.
View all availableELISA lysis buffers and reagents.
For Use With (Application): Western Blot, Sample preparation for ELISA
Product Type: Cell Extraction Buffer
Quantity: 100 ml
Shipping Condition: Dry Ice
Unit Size: Each