Waters, 176003598, XBridge Protein BEH SEC Column, 450 Å, 3.5 µm, 7.8 mm X 150 mm, 1/pk and BEH450 SEC Protein Standard Mix

XBridge Protein BEH SEC, 450 Å Columns, containing 2.5um particles are based on the same Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating as used in our successful line of HPLC-based SEC columns. They are best suited to separate proteins ranging from 100,000 – 1,500,000 Daltons. Note: This item in shipped with one vial the BEC450 SEC Protein Std  at no additional cost (PN 186006842).

Specifications
Chemistry: SEC
Separation Mode: Gel Filtration (aqueous)
Particle Substrate: Hybrid
pH Range Min: 2.5 pH
pH Range Max: 8 pH
Temperature Limits: 60 C
Maximum Pressure: 3000 psi (207 Bar)
Endcapped: No
Bonding Technology: Diol (OH)
Silanol Activity: Low
Molecular Weight Range Min: 100000
Molecular Weight Range Max: 1500000
Particle Shape: Spherical
Particle Size: 3.5 µm
Endfitting Type: Parker-style
Pore Size: 450 Å
QC Tested: Protein
Format: Online Column
Packing Solvent: 20% methanol in water
Surface Area: 80
System: UPLC
Particle Technology: BEH
Technique: LC
USP Classification: L33
Inner Diameter: 7.8 mm
Length: 150 mm
Carbon Load: 9 %
eCord: No
UNSPSC: 41115711
Application: Protein
Brand: XBridge
Product Type: Columns
Units per Package: 1 pk

Additional Information
XBridge Protein BEH SEC Column, 450 Å, 3.5 µm, 7.8 mm X 150 mm, 1/pk and BEH450 SEC Protein Standard Mix The Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating used in our successful series of HPLC-based SEC columns are employed in the XBridge Protein BEH SEC, 450 Å Columns, which contain 2.5µm particles. They are best suited for separating proteins with a size range of 100,000 to 1,500,000 Daltons, and The XBridge Protein BEH SEC Column and BEH450 SEC Protein Standard Mix were designed to meet even the most stringent chromatographic requirements. BEH base particle technology combined with diol bonding methods improves column stability, performance, and longevity. The XBridge Protein BEH SEC Column comes with a vial of the BEH450 SEC Protein Standard Mix. The BEH450 SEC Protein Standard Mix should be utilized as a benchmark for column and system performance. The diol-bonded covering of the XBridge Protein BEH SEC Column allows for improved flow and pressure tolerance for increased sample throughput on HPLC systems. The XBridge Protein BEH SEC Column facilitates the shift to UltraPerformance LC Technology and is designed to maximize productivity. Because it is manufactured in cGMP, ISO9001, and ISO13485 certified facilities, the XBridge Protein BEH SEC Column, like all Waters equipment, is dependable and reproducible. Every piece of equipment is thoroughly tested at every level and phase of manufacture to ensure the highest quality possible. Because of its unequaled column longevity and ability to perform with a wide range of mobile phase pH and temperatures, the column is an important piece of laboratory equipment. Visit our website to explore our complete line of XBridge columns and to shop for lab equipment that matches your individual needs. Please contact a member of our global team if you require assistance with any equipment or have any questions. You might also want to look into TruView LCMS Certified Clear Glass 12 x 32 mm Screw Neck Vial, with Cap and Preslit PTFE/Silicone Septum, 2 mL Volume, 100/pk ; TruView LCMS Certified vials are manufactured using highly controlled manufacturing and handling techniques. Mass spectrometers are used to check for cleanliness, and ACQUITY/MS/MS is used to check for low analyte adsorption.

FAQ
How Much Of A pH Range Can The XBridge Protein BEH SEC Column And BEH450 SEC Protein Standard Mix Operate Under? The XBridge Protein BEH SEC Column and BEH450 SEC Protein Standard Mix work well in the 2.5 - 8 pH range, at 60 C, and at a maximum pressure of 3000 psi (or 207 Bar). However, we do not recommend exceeding these pH, pressure, or temperature ranges because it may have an influence on the performance and durability of your column.