Waters, 186004860, XBridge BEH Amide Column, 130Å, 3.5 µm, 2.1 mm X 100 mm, 1/pk

XBridge BEH Amide columns retain extremely polar compounds, including carbohydrates and sugars. XBridge Amide columns offer you a chemically stable, trifunctionally bonded amide phase that is stable from pH 2-11 to enable the separation of polar analytes spanning a wide range of polarity, structural moiety and pKa.

Specifications
Chemistry: Amide
Separation Mode: Hydrophilic Interaction (HILIC)
Particle Substrate: Hybrid
pH Range Min: 2 pH
pH Range Max: 11 pH
Maximum Pressure: 6000 psi (415 Bar)
Endcapped: No
Silanol Activity: Low
Particle Shape: Spherical
Particle Size: 3.5 µm
Endfitting Type: Waters
Pore Size: 130 Å
Format: Column
Surface Area: 185
System: HPLC
Particle Technology: BEH
USP Classification: L68
Inner Diameter: 2.1 mm
Length: 100 mm
Carbon Load: 12 %
UNSPSC: 41115709
Brand: XBridge
Product Type: Columns
Units per Package: 1 pk

Additional Information
XBridge BEH Amide Column, 130Å, 3.5 µm, 2.1 mm X 100 mm, 1/pk Opt for the Waters XBridge BEH Amide column to retain extremely polar compounds, including carbohydrates and sugars. The use of the XBridge Amide analytical column provides you with a chemically stable, trifunctionally bonded amide phase that is stable across a wide pH range, making it possible for you to conduct the separation of polar analytes across a variety of features. As the XBridge BEH Amide Column is based on Waters’ novel ethylene bridged hybrid (BEH) particle, it makes use of a chemically stable, trifunctionally bonded amide phase to offer unprecedented stability and versatility in HILIC separations lab equipment. Waters has developed this ability as an innovative approach to overcoming the challenge of retaining and separating extremely polar compounds in HILIC stationary phases. The XBridge BEH Amide Column has the same selectivity as ACQUITY UPLC BEH Amide columns. This enables scientists to easily transfer separations seamlessly between HPLC and UPLC technology platforms. The XBridge BEH Amide Column is easily able to support the use of a wide range of mobile phase pH, from 2 to 11, as it has been designed specifically to retain polar analytes and metabolites that are too polar to retain using reversed-phase chromatography. This results in the exceptional retention of polar analytes spanning a wide range in polarity, structural moiety, and pKa. The XBridge BEH Amide Column listed here is particularly suited for the analysis of carbohydrates with the 3.5 µm particle size and 130Å pore size. This enables high resolution and high-speed analysis in complex sample matrices while maintaining or improving the chromatographic resolution. Refer to our product catalog or online store to shop for lab equipment that is authentic and available at the best available rates in the market. Waters recommends that you also invest in the XBridge BEH Amide VanGuard Cartridge, 130Å, 3.5 µm, 2.1 mm X 5 mm, 3/pk , so that your analytical column can last a long time and continue to deliver at its peak performance throughout its life.

FAQ
What Is The Importance Of Chromatography In Protein Analysis? In any proteomic analysis, the first and most important task is the separation of a complex protein mixture, i.e., the proteome. Chromatography is one of the most powerful methods of separation and employs one or more inherent characteristics of a protein-its mass, isoelectric point, hydrophobicity, or biospecificity.