Waters, 186006179, XSelect HSS PFP Column, 100Å, 2.5 µm, 3 mm X 100 mm, 1/pk

The XSelect HSS PFP column is a low coverage, unendcapped pentafluorophenyl phase that provides exceptional separation of closely related compounds as well as alternate selectivity to traditional alkyl chain columns due to an intricate combination of retention mechanisms. Packed in ultra-low dispersion hardware, 2.5 µm eXtended Performance [XP] columns enable exceptional separation performance, robustness and throughput for HPLC assays while enabling a seamless transition path toward UPLC adoption.

Specifications
Chemistry: PFP/Fluoro-Phenyl
Separation Mode: Reversed Phase
Particle Substrate: Silica
pH Range Min: 2 pH
pH Range Max: 8 pH
Maximum Pressure: 18000 psi (1240 Bar)
Endcapped: No
Bonding Technology: PFP
Silanol Activity: High
Particle Shape: Spherical
Particle Size: 2.5 µm
Endfitting Type: Parker-style
Pore Size: 100 Å
Format: Column
Surface Area: 230
System: UPLC, UHPLC
Particle Technology: HSS
USP Classification: L43
Inner Diameter: 3 mm
Length: 100 mm
Carbon Load: 7 %
eCord: Yes
UNSPSC: 41115709
Brand: XSelect
Product Type: Columns
Units per Package: 1 pk

Additional Information
XSelect HSS PFP Column, 100Å, 2.5 µm, 3 mm X 100 mm, 1/pk Due to a complex interplay of retention processes, the XSelect HSS PFP column, a low coverage, non-end-capped pentafluorophenyl phase, offers superior separation of chemically similar compounds as well as alternate selectivity to conventional alkyl chain columns. A seamless transition path to UPLC adoption is made possible by the inclusion of 2.5 m eXtended Performance [XP] columns in ultra-low dispersion hardware, which provides superior separation performance, resilience, and throughput for HPLC tests. One of the most reliable, repeatable, and scalable silica-based PFP LC columns on the market is the XSelect HSS PFP Column. Without compromising peak shape or retention time variation, the distinctive HSS substrate and PFP ligand combination offer real PFP performance. High Strength Silica (HSS) technology is used in the XSelect HSS PFP Column. With advantages like enhanced polar molecule retention and various selectivity, this technology was developed with chromatography in mind. In addition to its analytical capabilities, the HSS particle has the mechanical strength required to sustain UPLC pressures. The XSelect HSS PFP Column provides a special phase for selectivity for bases and planar aromatic compounds. When you require higher compound retention, especially for basic polar analytes, whose retention is challenging with C18 stationary phases, rely on the XSelect HSS PFP Column. PFP phases are electron acceptors; hence these ligands will interact pi-pi with Lewis base-containing molecules. Based on analyte form, dipole moment, and hydrogen bonding interactions, the stiff aromatic ring offers enhanced selectivity. The XSelect HSS PFP Column is available in different particle sizes and column formats to help you efficiently develop methods. You can browse through our website to shop for lab equipment and review the full list of Waters' product catalog. You might also be interested in XSelect HSS PFP XP VanGuard Cartridge, 100 Å, 2.5 µm, 2.1 mm X 5 mm, 3/pk. XSelect HSS PFP VanGuard Cartridges are used to extend analytical column lifetime and performance by removing particulate contamination from the mobile phase stream. This cartridge is optimized to protect all 2.1 mm and 3.0 mm I.D. XSelect HSS PFP analytical columns containing 2.5 µm sorbent particles.

FAQ
How Is Enrichment In Sample Components Present In Low Concentration Achieved? To enrich sample components that are present in low concentrations, several steps can be taken. To selectively elute analytes, you can modify the gradient steps. Utilizing "huge" sample quantities in an adsorption-promoting solvent is another efficient technique. Use a "small" collecting volume in a solvent that encourages desorption. Utilize sorbent chemistry specific to the analyte, rather than the chemistry found in the analytical column. It is important to carefully select the chemistry of the solid-phase extraction column to eliminate the need for additional sample preparation.