Waters, 186006595, XBridge BEH Amide Column, 130Å, 5 µm, 4.6 mm X 150 mm, 1/pk

XBridge BEH Amide columns retain extremely polar compounds, including carbohydrates and sugars. XBridge Amide columns offer you a chemically stable, trifunctionally bonded amide phase that is stable from pH 2 to 11 to enable the separation of polar analytes spanning a wide range of polarity, structural moiety and pKa.

Specifications
Chemistry: Amide
Separation Mode: Hydrophilic Interaction (HILIC)
Particle Substrate: Hybrid
pH Range Min: 2 pH
pH Range Max: 11 pH
Maximum Pressure: 6000 psi (415 Bar)
Endcapped: No
Silanol Activity: Low
Particle Shape: Spherical
Particle Size: 5 µm
Endfitting Type: Waters
Pore Size: 130 Å
Format: Column
Surface Area: 185
System: HPLC
Particle Technology: BEH
USP Classification: L68
Inner Diameter: 4.6 mm
Length: 150 mm
Carbon Load: 12 %
UNSPSC: 41115709
Brand: XBridge
Product Type: Columns
Units per Package: 1 pk

Additional Information
XBridge BEH Amide Column, 130Å, 5 µm, 4.6 mm X 150 mm, 1/pk The XBridge BEH Amide Column was specifically designed to retain polar analytes and metabolites that are too polar to retain via regular reversed-phase chromatography. With a pH range for the mobile stream of 2 to 11, the XBridge BEH Amide Column will provide you exceptional retention for polar analytes spread over a wide variety of structural moiety, pKa, and polarity, making it an excellent piece of lab equipment for you to have. The XBridge BEH Amide Columns are based on Waters’ Ethylene Bridged Hybrid (BEH) particle technology that provides a chemically stable, tri-functionally bonded amide phase which produces stability and versatility in HILIC separations. This XBridge BEH Amide Column, listed here, is particularly well suited for running analysis of carbohydrates with the 5 µm particle size, 130 pore size, and a carbon load of 12%. This enables high resolution and high-speed analysis even within complex sample matrices, while maintaining or improving the overall chromatographic resolution. Designed for maximum efficiency and productivity, the XBridge BEH Amide Columns have the same selectivity as the ACQUITY UPLC BEH Amide Column, which would enable you to have seamless transfer separations between HPLC and UPLC technology platforms. The analytical column is manufactured in the dedicated Waters chromatography chemistry manufacturing plant under the strictest cGMP, ISO 9002 conditions. At the manufacturing plant, silica-based and polymeric chromatography materials are synthesized and packed into columns and solid-phase extraction devices. This ensures that you get the highest quality lab equipment that delivers consistent and reliable analysis results. You get the most critical performance features when you purchase the XBridge BEH Amide Column: column efficiency, peak symmetry, column lifetime, and column-to-column reproducibility. Shop for lab equipment so you can replenish your stock and add to your existing equipment portfolio; you can also browse our website and refer to our catalog. Along with the XBridge BEH Amide Columns, we also recommend you check out the XBridge BEH Amide VanGuard Cartridge, 130Å, 5 µm, 3.9 mm X 5 mm, 3/pk . This cartridge is designed for extending analytical column lifetime and performance by removing particulate contamination from the mobile phase stream. The XBridge BEH Amid VanGuard cartridge is optimized to protect all 4.6 mm I.D. XBridge BEH Amide analytical columns containing 5 µm sorbent particles.

FAQ
What Is The Importance Of Resolution In Chromatography? Resolution, a crucial parameter in chromatography, can be optimized by considering two intrinsic factors of the chromatographic process: peak width and retention time. Peak width represents the spread of a chromatographic peak, while retention time indicates the duration a component spends in the stationary phase. By manipulating these factors, the resolution between adjacent peaks can be improved, leading to enhanced separation of analytes in a mixture. Therefore, resolution measurements serve as a valuable tool for fine-tuning and optimizing separations, enabling researchers to achieve superior analytical performance and accuracy in chromatographic analysis.