Waters, 186008801, nanoEase M/Z Peptide BEH C18 Column, 300Å, 1.7 µm, 75 µm X 250 mm, 1/pk

nanoEase M/Z Peptide BEH C18 Column, 300Å, 1.7 µm, 75 µm X 250 mm columns are designed for optimal performance for nano- to micro scale liquid chromatographic separation, offering the highest possible sensitivity, resolution and reproducibility for biomarker discovery and protein identification and characterization. Like all M/Z columns they withstand 15kpsi operating pressure and handle pressure fluctuations during injection extremely well and thus provide superior longevity. In order to achieve maximum performance, it is important to ensure that proper connections are made to minimize peak tailing and poor efficiency. This specific offering contains Waters patented BEH Technology 300Å pore, C18, 1.7 µm particles. The wide-pore (300Å) trifunctionally bonded BEH particle offers the widest usable pH range, superior low pH stability, and ultra-low column bleed to assay samples for especially large peptides in proteomics, protein characterization, and peptide synthesis. Each batch is specifically QC tested using a gradient separation of a tryptic digest of cytochrome c (p/n: 186006371) to before being QC accepted as a Peptide separation column. The columns are equipped with the ZenFit connector, an easy to use, fingertight, tool free liquid connection. This ensures reproducible, leak free connections. The fittings will fit into Waters V-Detail ports as well as into Rheodyne/Vici compatible valves. Suggested Uses: Bottom-up Proteomics, Nano-flow, Trap and Elute

Specifications
Chemistry: C18
Separation Mode: Reversed Phase
Particle Substrate: Hybrid
pH Range Min: 1 pH
pH Range Max: 10 pH
Maximum Pressure: 15000 psi (1034 Bar)
Endcapped: Yes
Silanol Activity: Low
Particle Shape: Spherical
Particle Size: 1.7 µm
Endfitting Type: 10-32 for 1/16" male /360um open
Pore Size: 300 Å
QC Tested: Peptide
Format: Column
Surface Area: 90
System: Nano/Micro
Particle Technology: BEH
USP Classification: L1
Inner Diameter: 75 µm
Length: 250 mm
Carbon Load: 12 %
UNSPSC: 41115711
Application: Peptide
Brand: nanoEase M/Z
Product Type: Columns
Units per Package: 1 pk

Additional Information
nanoEase M/Z Peptide BEH C18 Column, 300Å, 1.7 µm, 75 µm X 250 mm, 1/pk Use the nanoEase M/Z Peptide BEH C18 Column for the best results in liquid chromatographic separation at the nano- to microscale. The lab equipment offers the best sensitivity, resolution, and repeatability for biomarker discovery and protein identification and characterization. The nanoEase M/Z Peptide BEH C18 Column version below uses Waters' proprietary 300 A pore, C18, 1.7 µm particles. Making sure appropriate connections are made to prevent peak tailing and wasteful operation helps to achieve optimal performance. In order to guarantee that you only receive the best lab equipment, each batch of the nanoEase M/Z Peptide BEH C18 Column is QC verified utilizing a gradient separation of a tryptic digest of cytochrome c. The nanoEase M/Z Peptide BEH C18 packing materials were developed specifically for use with the ACQUITY UPLC System and are manufactured in a cGMP, ISO 9001-certified facility using ultra-pure reagents. Each batch of nanoEase M/Z Peptide BEH C18 packing material is chromatographically tested with acidic, basic, and neutral analytes, with the results constrained to narrow specification ranges to assure exceptional, consistent performance. Each column is individually evaluated, and an eCordTM Intelligent Chip is used to produce a Performance Chromatogram and a Certificate of Batch Analysis. The nanoEase M/Z Peptide BEH C18 Column's pre-assembled ZenFit Connectors are made to fit into connection ports with a V-detail appearance. The V-detail connection port is a 10-32 threaded port for 1/16" O.D. tubing that is simple to use, reusable, fingertight, and can withstand pressures at 90°C. This makes it easier to construct crucial nano- and microflow UPLC fluid connections. Using the nanoEase M/Z Peptide BEH C18 Column, flow-path-induced variability in chromatographic results may be effectively eliminated. You may also want to explore the Neutrals QC Reference Material . It contains one ampule with 10 μL/mL acetone, 0.25 mg/ mL naphthalene, and 0.4 mg/ mL acenaphthene in 50/50 acetonitrile/water, 2 mL. The Neutrals QC Reference Material can be used with any chromatographic system that has a UV detector. It is meant to be used as a benchmarking standard for systems. Only neutral chemicals are present in the mixture.

FAQ
Across What pH Range Does The nanoEase M/Z Peptide BEH C18 Column Operate? The nanoEase M/Z Peptide BEH C18 Column listed here is able to conduct analysis within the 1-10 pH range. What Carbon Load Is Offered By The nanoEase M/Z Peptide BEH C18 Column? This particular nanoEase M/Z Peptide BEH C18 Column carries a carbon load of 12%.