{"product_id":"abcam-ab100578","title":"Abcam, ab100578, Human Insulin ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Insulin ELISA kit (colorimetric) is a sandwich ELISA designed to quantify Human Insulin (INS) and Proinsulin with a sensitivity of \u0026lt;4 µIU\/mL.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:\u0026lt; 4 µlU\/mL,\u003cbr\u003e\nRange:4.69 - 300 µlU\/mL,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Insulin ELISA Kit ab100578 is a sandwich ELISA to measure human Insulin (INS) and Proinsulin in serum, plasma, and cell culture supernatant with a sensitivity of \u0026lt;4 µIU\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nThis assay employs an antibody specific for Human Insulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Insulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Insulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Insulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003cbr\u003e\nAssay specificity\u003cbr\u003e\nThis kit recognizes Insulin and Proinsulin.\u003cbr\u003e\nCross reactivity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human BDNF, BLC, ENA-78, IL-1α, IL-1β, IL-2, IL-3, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF.\u003cbr\u003e\nHuman Insulin ELISA Kit ab100578 protocol summary\u003cbr\u003e\n1. Add samples and standards to wells. Incubate at room temperature.\u003cbr\u003e\n2. Wash each well and add biotinylated antibody. Incubate at room temperature.\u003cbr\u003e\n3. Wash each well and add Streptavidin Solution. Incubate at room temperature.\u003cbr\u003e\n4. Add TMB Solution to each well. Incubate at room temperature.\u003cbr\u003e\n5. Add Stop Solution to each well. Read immediately.\u003cbr\u003e\nHow other researchers are using Human Insulin ELISA Kit ab100578\u003cbr\u003e\nHuman Insulin ELISA Kit ab100578 has been used in publications to study the effects of NRG1 in children with obesity\u003cbr\u003e\nand therapeutic effects of mesenchymal stem cells in diabetes\u003cbr\u003e\nReferences:\u003cbr\u003e\nJohnny D Figueroa et al. 2022 PMID: 35220393,\u003cbr\u003e\nYongsung Hwang et al. 2023 PMID: 37644502\u003cbr\u003e\nGet results in 90 minutes our SimpleStep ELISA\u003cbr\u003e\n- Human Insulin ELISA Kit\u003cbr\u003e\nab278123\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nHuman Insulin ELISA Kit ab100578 is commonly used to study diabetes and insulin disorders.\u003cbr\u003e\nOther kits for measuring metabolic health include:\u003cbr\u003e\n- Human IgG ELISA Kit\u003cbr\u003e\nab195215\u003cbr\u003e\n- Human Transferrin ELISA Kit\u003cbr\u003e\nab288174\u003cbr\u003e\n- Human Glutathione Peroxidase 4 ELISA Kit\u003cbr\u003e\nab304936\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nInsulin a peptide hormone plays an essential mechanical role in carbohydrate and fat metabolism. Insulin alternatively known as bovine insulin in some research contexts has a molecular mass of around 5800 Da. Beta cells in the pancreas express insulin where it gets secreted directly into the bloodstream. The hormone regulates glucose levels by facilitating the uptake of glucose into cells such as muscle and adipose tissues.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nInsulin influences several key metabolic processes by binding to its receptor a component of the insulin receptor substrate family. This triggers a cascade of events within cells promoting the uptake of glucose and converting it into energy. Insulin operates as a singular entity but plays a critical role in forming a complex with its receptor. This interaction triggers downstream effects that alter the activity of enzymes and transcription factors involved in glucose and lipid metabolism.\u003cbr\u003e\nPathways\u003cbr\u003e\nInsulin is critical for the regulation of the PI3K-AKT signaling pathway and the MAPK pathway. These pathways modulate processes such as cell growth proliferation and survival. Insulin interacts closely with other proteins in these pathways such as the insulin receptor substrate proteins and influences downstream proteins like AKT kinase which has a significant role in mediating the metabolic effects initiated by insulin binding.\u003cbr\u003e\nInsulin is most notably linked to diabetes mellitus characterized by insufficient insulin production or action. This deficiency disrupts normal glucose homeostasis leading to hyperglycemia. Other associated proteins include glucagon which counteracts insulin's role in lowering blood glucose levels and IRS-1 an important element through which insulin signaling is transduced. Researchers utilize mouse insulin ELISA and insulin ELISA kits to better understand these relationships and develop treatments for insulin-related disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843544666281,"sku":"ab100578","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab100578","provider":"Iright","version":"1.0","type":"link"}