{"product_id":"abcam-ab100647","title":"Abcam, ab100647, Human TGF beta 1 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman TGF beta 1 ELISA kit (colorimetric) is a sandwich ELISA designed to quantify transforming growth factor beta 1 (TGF-β 1) with a sensitivity of 18 pg\/mL. - Wide dynamic range – quantifies 18–4,000 pg\/mL - Broad sample compatibility – works with serum, plasma, and cell culture supernatants - Cited in over 85 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 18 pg\/mL,\u003cbr\u003e\nRange:18 - 4000 pg\/mL,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman TGF beta 1 ELISA Kit ab100647 is a sandwich ELISA to measure human TGF beta 1 (TGF-β1) in serum, plasma, and cell culture supernatant with a sensitivity of 18 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nThis assay employs an antibody specific for Human TGF beta 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TGF beta 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TGF beta 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TGFB 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003cbr\u003e\nAssay specificity\u003cbr\u003e\nCross-reactivity:\u003cbr\u003e\nThis ELISA kit shows no cross-reactivity with any of the cytokines tested; ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO-α, GRO-β, GRO-γ, I-309, IFN-γ, IL-1α, IL-1β, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1α, MIP-1β, NAP-2, PDGF, PF-4, PARC, SCF, SDF-1α, TIMP-1, TIMP-2, TNFβ, TGFβ2, TGFβ3, VEGF.\u003cbr\u003e\nHuman TGF beta 1 ELISA Kit ab100647 protocol summary\u003cbr\u003e\n1. Add samples and standards to wells. Incubate at room temperature\u003cbr\u003e\n2. Wash each well and add biotinylated antibody. Incubate at room temperature\u003cbr\u003e\n3. Wash each well and add Streptavidin Solution. Incubate at room temperature\u003cbr\u003e\n4. Add TMB Solution to each well. Incubate at room temperature\u003cbr\u003e\n5. Add Stop Solution to each well. Read immediately\u003cbr\u003e\nHow other researchers are using Human TGF beta 1 ELISA Kit ab100647\u003cbr\u003e\nHuman TGF beta 1 ELISA Kit ab100647 has been used to study inflammation in different physio-pathological contexts such as squamous cell carcinoma\u003cbr\u003e\n, gastric cancer\u003cbr\u003e\n, and osteoarthritis\u003cbr\u003e\nReferences:\u003cbr\u003e\nYi Huang et al. 2023, PMID: 37872157,\u003cbr\u003e\nShuai Lv et al. 2024, PMID: 38490994,\u003cbr\u003e\nZhong Wu, et al. 2023, PMID: 37842584\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nHuman TGF beta 1 ELISA Kit ab100647 is commonly used to measure IFN gamma as a marker of inflammation.\u003cbr\u003e\nOther ELISA kits to measure inflammatory markers include:\u003cbr\u003e\n- Human IL-6 ELISA kit\u003cbr\u003e\nab178013\u003cbr\u003e\n- Human IL-10 ELISA Kit (Interleukin-10)\u003cbr\u003e\nab185986\u003cbr\u003e\n- Human TNF alpha ELISA kit\u003cbr\u003e\nab181421\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843539783849,"sku":"ab100647","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab100647","provider":"Iright","version":"1.0","type":"link"}