{"product_id":"abcam-ab100733","title":"Abcam, ab100733, Mouse Osteoprotegerin ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nMouse Osteoprotegerin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse Osteoprotegerin in Mouse in Biofluids, Cell Culture Media samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:\u0026lt; 1 pg\/mL,\u003cbr\u003e\nRange:1.65 - 1200 pg\/mL,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAbcam's Osteoprotegerin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an\u003cbr\u003e\nin vitro\u003cbr\u003e\nenzyme-linked immunosorbent assay for the quantitative measurement of mouse Osteoprotegerin in serum, plasma and cell culture supernatants.\u003cbr\u003e\nThis assay employs an antibody specific for mouse Osteoprotegerin coated on a 96-well plate. Standards and samples are pipetted into the wells and Osteoprotegerin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Osteoprotegerin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Osteoprotegerin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nOsteoprotegerin (OPG) also known as osteoclastogenesis inhibitory factor is a glycoprotein with a mass of approximately 60 kDa. It acts as a soluble decoy receptor for receptor activator of nuclear factor kappa-Β ligand (RANKL) and is encoded by the TNFRSF11B gene. OPG is mainly expressed in osteoblasts fibroblasts and endothelial cells. Its expression can be found in diverse tissues throughout the body including the liver and lungs.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nOPG inhibits the differentiation and activation of osteoclasts by binding to RANKL preventing its interaction with the receptor RANK on the surface of osteoclast progenitor cells. As part of a non-classical signaling pathway OPG plays a significant role in the regulation of bone remodeling and maintenance by controlling bone resorption. This activity preserves bone density and structure by limiting excessive osteoclastic activity.\u003cbr\u003e\nPathways\u003cbr\u003e\nOPG operates within the RANK\/RANKL\/OPG signaling axis which is important for bone metabolism. It counteracts the bone-resorption pathway driven by the RANKL-RANK interaction inhibiting osteoclastogenesis. This axis involves interactions with other proteins such as RANK RANKL and TRAIL where TRAIL’s involvement hints towards a broader immune regulation role for OPG.\u003cbr\u003e\nOPG has a significant association with conditions such as osteoporosis and vascular calcification. In osteoporosis an imbalance in the RANKL\/OPG ratio can lead to excessive bone loss due to increased osteoclast activity. In vascular calcification OPG appears to play a protective role likely linked with regulatory mechanisms involving RANKL and TRAIL. Understanding these interactions opens potential therapeutic avenues particularly in managing bone-related and cardiovascular conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843628945577,"sku":"ab100733","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab100733","provider":"Iright","version":"1.0","type":"link"}